Composite

Part:BBa_K2242521:Design

Designed by: YongHao Liang   Group: iGEM17_USTC   (2017-10-11)


placI+lacI+pTAC+CmCR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3539


Design Notes

No.


Source

The plasmid backbone pYYDT is kindly provided by Prof. Yu Hanqing. The gene CmCR is kindly provided by Prof. Hong Jiong. The recombinant plasmid is constructed by ourselves.

References